I got the same error form what seemed out of the blue, I created a runtime file one day and the very next day made another change and I the error when I went to do another runtime file “ERROR: Unsupported feature=Audit Trail; Component type=Startup Validation completed with errors. The runtime application has NOT been created.”
Running in compatibility mode worked for me. The only thing that changed from on day to another what I think was a mandatory update from the company I work for. It wasn’t a windows update not sure exactly what it was.
Anyway thanks for the great information.